Ultra-wide field functional imaging becomes quite popular nowadays. More and more scientists want to image on large field of view and single-cell resolution at the same time. Unfortunately, there are some serious tradeoffs among those desires. A Japanese team claims they solved these optical, mechanical, and biological problems. So here are the facts: 3x3 mm FOV, over 16000 identified neurons, 7.5 Hz sampling rate and excellent SNR. These novel engineering solutions might help us to see the bigger picture in neuroscience!
The original study was uploaded to bioRxiv.
Conventional objective lens (CFI Plan Fluor 10X/0.30, Nikon) and newly developed large objective, tube and scan lenses.