How can we understand better complex neural processes such as dendritic integration and encoding? The objects are always in a volume not just in one single plane and we can not see the bigger picture without recording the whole volume. In a recent paper Dr. Sakaki and his colleagues developed a two-photon microscope which using acousto-optic deflectors for ultrafast 3D scanning. It enables random-access sampling of thousands of points-of-interest. They showed nice examples on tectal neurons in albino Xenopus laevis tadpoles brain. They used single-cell electroporation for expression of a red space-filling fluorophore to determine dendritic arbor morphology, and either the calcium sensor jGCaMP7s or the glutamate sensor iGluSnFR as indicators of neural activity. These cutting-edge techniques allow examining complex input-output patterns within an intact brain.
Excellent electroporators can be also find here at #NeuroRigBuilder: link