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Ex vivo and in vitro applications / Slice perfusion components / Chambers
Dual-Superfusion Slice Chamber ST
It is a unique, worldwide patented idea to support extended survival of brain slices. The Dual-superfusion Slice Chamber Insert is recommended for all types of slice recordings, but it is indispensable for investigations where oxygen is a limiting factor of neuronal function, e.g. network oscillations (Hajos et al., European Journal of Neuroscience, Vol. 29, pp. 319-327, 2009).
Dual superfusion of submerged slices increases the vitality of cells as oxygen, as well as other materials. They can freely and more effectively diffuse into the thick slices from both sides. In the most widely used slice chambers, where the slices are sitting on a glass cover slip, oxygenation and drug diffusion is achieved only from one side.
A chamber insert was created where slices sit on a mesh, and the shape of the insert supports laminar flow around the submerged slices ensuring better oxygenation. The mesh divides the chamber into two parts. Both the upper (above the mesh), and the lower (below the mesh) parts of the chamber are 2 mm in height. By using this type of chamber design the flow rate of the superfused solutions can be kept as low as 3-5 ml/min, while the oxygenation of the tissue can be maximally maintained.
The Dual-superfusion Slice Chamber Insert is 20 mm in outer diameter. This diameter was chosen because this way our product is compatible with the most popular brain slice chambers on the market. Bigger meshes will be introduced later in the same 20 mm outer diameter for human brain slices. Measurements of your existing brain slice chamber to be compatible with the Dual-superfusion Slice Chamber Insert are:
- the diameter of the internal center hole must be larger than 20 mm
- the effective depth of the internal center hole should be approximately 5 mm or more
Specializtion and technical data:
The working (meshed) area: 7.2 by 7.2 mm
Fields of applications:
in vitro slice measurements, thick slice technic
Descriptions
It is a unique, worldwide patented idea to support extended survival of brain slices. The Dual-superfusion Slice Chamber Insert is recommended for all types of slice recordings, but it is indispensable for investigations where oxygen is a limiting factor of neuronal function, e.g. network oscillations (Hajos et al., European Journal of Neuroscience, Vol. 29, pp. 319-327, 2009).
Dual superfusion of submerged slices increases the vitality of cells as oxygen, as well as other materials. They can freely and more effectively diffuse into the thick slices from both sides. In the most widely used slice chambers, where the slices are sitting on a glass cover slip, oxygenation and drug diffusion is achieved only from one side.
A chamber insert was created where slices sit on a mesh, and the shape of the insert supports laminar flow around the submerged slices ensuring better oxygenation. The mesh divides the chamber into two parts. Both the upper (above the mesh), and the lower (below the mesh) parts of the chamber are 2 mm in height. By using this type of chamber design the flow rate of the superfused solutions can be kept as low as 3-5 ml/min, while the oxygenation of the tissue can be maximally maintained.
The Dual-superfusion Slice Chamber Insert is 20 mm in outer diameter. This diameter was chosen because this way our product is compatible with the most popular brain slice chambers on the market. Bigger meshes will be introduced later in the same 20 mm outer diameter for human brain slices. Measurements of your existing brain slice chamber to be compatible with the Dual-superfusion Slice Chamber Insert are:
- the diameter of the internal center hole must be larger than 20 mm
- the effective depth of the internal center hole should be approximately 5 mm or more
Specializtion and technical data:
The working (meshed) area: 7.2 by 7.2 mm
Fields of applications:
in vitro slice measurements, thick slice technic
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