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Fiber Photometry System
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Fiber Photometry System

RWD Fiber Photometry System aims to detect the activity change of neurons in the central nervous system in real-time, the fiber photometry sums up the overall fluorescence of neurons to express their overall activity without reflecting on the activity of individual neurons. It is a multi-channel, dual-color device.

Based on the reference channel of 410 nm wavelength, RWD Fiber Photometry System adopts 470 nm and 560 nm dual-wavelength excitation reducing the background noise effectively. The significance of the reference channel lies in increasing self-control, reducing spontaneous fluorescence, eliminating signal artifacts caused by animal's intense exercise and fiber winding, expressing two kinds of proteins at the same site, detecting levels of calcium change in different groups of neurons, and truly realizing the recording and analysis of dual-color fluorescence signals.

Features:

  • With 410 & 470 & 560 three kinds of LED excitation light source, of which 410 is the reference channel, the fluorescence signal acquisition is more accurate.
  • It can support up to 7 channels, suitable for high-throughput calcium signal acquisition or simultaneous recording of multiple neural nucleus groups.
  • When the external event is marked, the time automatically inputs to achieve synchronous recording with behavioral video, and the external stimulus time synchronization accuracy is up to 10ms.
  • The software presets four LED excitation output modes, which can be applied to different experimental application scenarios.
  • High performance and lowThe noisy CMOS camera can collect calcium fluorescence signals of multiple channels and multiple wavelengths at the same time.
  • Integrated output port and pull-top cover design make the experimental fluorescence signal path less disturbed by the external environment and easy to operate.
Parameter item  Specification
Frame Rate of CMOS 5~40 FPS
CameraCMOS camera resolution 1920*1200
Megapixels 2.3 MP
Exposure time setting range 1~100 ms
Fluorescence band 499-529 nm 581-618 nm
Central wavelength of excitation light 410 nm、470 nm、560 nm
LED power-adjustable percentage 0~100%
Fluorescence detection mode Four modes are optional, i.e. CNST、TRG1、TRG2、TRG3
410nm LED Output Power Range 0~300 μW
470nm LED Output Power Range 0~180 μW
560nm LED Output Power Range 0~90 μW
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Descriptions

Based on the reference channel of 410 nm wavelength, RWD Fiber Photometry System adopts 470 nm and 560 nm dual-wavelength excitation reducing the background noise effectively. The significance of the reference channel lies in increasing self-control, reducing spontaneous fluorescence, eliminating signal artifacts caused by animal's intense exercise and fiber winding, expressing two kinds of proteins at the same site, detecting levels of calcium change in different groups of neurons, and truly realizing the recording and analysis of dual-color fluorescence signals.

Features:

  • With 410 & 470 & 560 three kinds of LED excitation light source, of which 410 is the reference channel, the fluorescence signal acquisition is more accurate.
  • It can support up to 7 channels, suitable for high-throughput calcium signal acquisition or simultaneous recording of multiple neural nucleus groups.
  • When the external event is marked, the time automatically inputs to achieve synchronous recording with behavioral video, and the external stimulus time synchronization accuracy is up to 10ms.
  • The software presets four LED excitation output modes, which can be applied to different experimental application scenarios.
  • High performance and lowThe noisy CMOS camera can collect calcium fluorescence signals of multiple channels and multiple wavelengths at the same time.
  • Integrated output port and pull-top cover design make the experimental fluorescence signal path less disturbed by the external environment and easy to operate.
Parameter item  Specification
Frame Rate of CMOS 5~40 FPS
CameraCMOS camera resolution 1920*1200
Megapixels 2.3 MP
Exposure time setting range 1~100 ms
Fluorescence band 499-529 nm 581-618 nm
Central wavelength of excitation light 410 nm、470 nm、560 nm
LED power-adjustable percentage 0~100%
Fluorescence detection mode Four modes are optional, i.e. CNST、TRG1、TRG2、TRG3
410nm LED Output Power Range 0~300 μW
470nm LED Output Power Range 0~180 μW
560nm LED Output Power Range 0~90 μW

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