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In vivo applications / Behavioural applications
OpenSpritzer with LCD
The NeuroGig Openspritzer
The ability to precisely deliver picolitre volumes has always been a vital part of Biological research, until now this has required a significant investment of up to £4,500 into an established brand.
The Openspritzer (developed, described and published by Forman et al., 2017) serves as an alternative open source micro-ejection/puffer system with similar performance and reliability that you would expect from leading brands, but at a fraction of the cost. NeuroGig has done some modifications to the design as seen in the mock-up image above.
As with the more familiar models on the market, the Openspritzer is a self-contained system that has been designed to be intuitive, with simple functionality. Featuring a built-in ejection control system which is easily adjusted using a rotary switch you have complete control of the ejection duration; you also have the option of controlling the duration via a remotely generated TTL pulse. Ejection pressure is adjusted via the built-in regulator along with an accurate monitoring gauge. The Openspritzer is also supplied with a foot switch for added ease of operation.
Openspritzer versus other commercially available instruments
- A lateral offset between normalised peak intensities, where e.g. the Picospritzer can deliver smaller doses for a given puff duration. This difference could also be due to small differences in pressure - whilst both instruments were set to 1.4 bar, there could be internal pneumatic differences in the complete systems.
- Variability of the dosage on repeated puffs. The Openspritzer sometimes delivers slightly higher doses at the same settings as the Picospritzer, but this could also be an artefact. Openspritzer puffs were set at one second delays in between pulses whilst Picospritzer pulses were set and triggered manually.
- Absence of intermediate pressure to prevent back filling in the off state. This function could easily be added with another regulator at port 3 of the solenoid valve.
- Vacuum holding capacity to hold cells in place. This could easily be accomplished with another Openspritzer in tandem.
- Complete access to the source code for the control of the Openspritzer. This provides users with an increased range of parameters for controlling timing and puff duration.
Openspritzer Specifications
Valve Type - 114mA Fast switching Solenoid (MHE2-MS1H-32G-M7)
Power supply - 24V DC supply for solenoid valve
Control - 1mA/5V Internally generated pulses from Arduino Nano microcontroller (Externally delivered TTL pulses via BNC connector)
Foot switch - Yes
Air regulator - Festo LRP-1/4-4
TTL trains - Yes, at 10Hz TTL train with altered line pressure generated action potentials, but not at 2 or 5Hz (single puff of glutamate insufficient to activate action potential)
Shortest command pulse - 2ms (to reliably generate a detectable puff, in comparison with 4ms for Picospritzer)
Puff pressure - 0-4 bar
Line pressure - 1.4 bar
Precision - Millisecond precision with highly conserved puff volumes
Descriptions
The NeuroGig Openspritzer
The ability to precisely deliver picolitre volumes has always been a vital part of Biological research, until now this has required a significant investment of up to £4,500 into an established brand.
The Openspritzer (developed, described and published by Forman et al., 2017) serves as an alternative open source micro-ejection/puffer system with similar performance and reliability that you would expect from leading brands, but at a fraction of the cost. NeuroGig has done some modifications to the design as seen in the mock-up image above.
As with the more familiar models on the market, the Openspritzer is a self-contained system that has been designed to be intuitive, with simple functionality. Featuring a built-in ejection control system which is easily adjusted using a rotary switch you have complete control of the ejection duration; you also have the option of controlling the duration via a remotely generated TTL pulse. Ejection pressure is adjusted via the built-in regulator along with an accurate monitoring gauge. The Openspritzer is also supplied with a foot switch for added ease of operation.
Openspritzer versus other commercially available instruments
- A lateral offset between normalised peak intensities, where e.g. the Picospritzer can deliver smaller doses for a given puff duration. This difference could also be due to small differences in pressure - whilst both instruments were set to 1.4 bar, there could be internal pneumatic differences in the complete systems.
- Variability of the dosage on repeated puffs. The Openspritzer sometimes delivers slightly higher doses at the same settings as the Picospritzer, but this could also be an artefact. Openspritzer puffs were set at one second delays in between pulses whilst Picospritzer pulses were set and triggered manually.
- Absence of intermediate pressure to prevent back filling in the off state. This function could easily be added with another regulator at port 3 of the solenoid valve.
- Vacuum holding capacity to hold cells in place. This could easily be accomplished with another Openspritzer in tandem.
- Complete access to the source code for the control of the Openspritzer. This provides users with an increased range of parameters for controlling timing and puff duration.
Openspritzer Specifications
Valve Type - 114mA Fast switching Solenoid (MHE2-MS1H-32G-M7)
Power supply - 24V DC supply for solenoid valve
Control - 1mA/5V Internally generated pulses from Arduino Nano microcontroller (Externally delivered TTL pulses via BNC connector)
Foot switch - Yes
Air regulator - Festo LRP-1/4-4
TTL trains - Yes, at 10Hz TTL train with altered line pressure generated action potentials, but not at 2 or 5Hz (single puff of glutamate insufficient to activate action potential)
Shortest command pulse - 2ms (to reliably generate a detectable puff, in comparison with 4ms for Picospritzer)
Puff pressure - 0-4 bar
Line pressure - 1.4 bar
Precision - Millisecond precision with highly conserved puff volumes
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