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Ex vivo and in vitro applications / Slice perfusion components
Dual perfusion chamber is used for live-cell imaging. It is designed to maintain isolated, living tissues in healthy state during experiments. In contrast with the single perfusion, dual-superfusion of submerged slices increases the vitality of cells, since oxygen, as well as other materials, can freely and effectively diffuse with high flow rate from both sides into thick slices (up to 800 µm). Using dual perfusion chamber kit, whole-cell patch-clamp and field recordings can be performed simultaneously, physiological network oscillations such as sharp waves ripples (SPWs) are more preserved, furthermore, increased network activity may generate dendritic regenerative activities.
Kit contains:
• Unique dual perfusion polycarbonate chamber
• Slice holder
• Bubble trap
• Dual-channel in-line solution heater
• Temperature controller
• Peristaltic pump
Features
- Perfusion form both tissue sides
- Usable slices with up to 800 µm thickness
- Continuous and smooth fluid flow
- Adjustable, stable temperature
- Fluid degassing and debubbling
Benefits
- Increased oxygenation and vitality of cells
- Maintain acute, thick slice preparations
- Preserved physiological network oscillations during neurophysiological studies
Descriptions
Dual perfusion chamber is used for live-cell imaging. It is designed to maintain isolated, living tissues in healthy state during experiments. In contrast with the single perfusion, dual-superfusion of submerged slices increases the vitality of cells, since oxygen, as well as other materials, can freely and effectively diffuse with high flow rate from both sides into thick slices (up to 800 µm). Using dual perfusion chamber kit, whole-cell patch-clamp and field recordings can be performed simultaneously, physiological network oscillations such as sharp waves ripples (SPWs) are more preserved, furthermore, increased network activity may generate dendritic regenerative activities.
Kit contains:
• Unique dual perfusion polycarbonate chamber
• Slice holder
• Bubble trap
• Dual-channel in-line solution heater
• Temperature controller
• Peristaltic pump
Features
- Perfusion form both tissue sides
- Usable slices with up to 800 µm thickness
- Continuous and smooth fluid flow
- Adjustable, stable temperature
- Fluid degassing and debubbling
Benefits
- Increased oxygenation and vitality of cells
- Maintain acute, thick slice preparations
- Preserved physiological network oscillations during neurophysiological studies
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