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NINscope Kit
https://www.neurorigbuilder.com #NeuroRigBuilder

NINscope Kit

from Neurogig

The NINscope is a head-mountable, miniaturized microscope. This is a complete NINscope kit for self assembly. Includes all parts required to assemble a NINscope.

The NINscope

Creative tinkerers and developers, along with recent advances in optogenetics, genetically-encoded Calcium indicators and miniaturized optics and electronics made head-mountable microscopes a popular tool. Innovations from the founders of Inscopix and the UCLA team with their Miniscope have made this technology more accessible.

The NINscope (developed and published by de Groot et al., 2019 from the Netherlands Institute for Neuroscience) is a more recent development. It is a  smaller footprint, light weight hybrid of the Miniscope, with several other interesting integrated features. It is ideal for use on small freely behaving animals, or when two simultaneous recording areas is of interest, since it is small enough for a mouse to have two NINscope's mounted at the same time.  

NINscope design and features

For more information on miniscope designs and features, please read further in the publication by de Groot et al., 2019. They described their instrument design, manufacturing methods and functionality in detail here.

NINscope Specifications

Weight - 1.6g (Yes, this includes the housing, optics & PCBs!)

Footprint -6.5 x 7.5 mm baseplate 

Sensor - CMOS 752x480 pixels (Python480)  with Kyocera CMOS clock oscillator  

Pixel Size - 4.8 µm  

Inertial Measurement Unit - Yes, 3D accelerometer (STMicoreectronics LSM6DSLTR) 

LED Driver - Yes, 2 LED drivers and 12C I/O expander  

Accuracy -1 ms 

Optogenetic stimulation LED probe - Yes, custom probe 

Multi-region recordings - Yes 

Inter-baseplate distance - 8.15 mm 

Wireless - Yes

Coaxial Cable - CMOS connects via FPD-Link III and coaxial to USB controller on the DAQ board 

Excitation/Emission Filters size - 3.5x3.5x0.5 mm Excitation/ 4x4x1 mm Emission 

Dichroic Mirror - Chroma T495lpxr  (3.5x5x0.5 mm) 

GRIN lens - Yes, #64-519 Edmund Optics Grin lens with 1.8 mm diameter *implanted or glued into baseplate 

Numerical Aperture - 0.55

Magnification - 4.6x

DAQ - UCLA Miniscope DAQ card with minor modifications

Time per recording session - 2-11 min with brief intermissions 

Photobleaching/damage - None evident

Enclosure - 3D printed

Open Source Software - Yes, platform independent user interface with two channels for recordings and an additional USB webcam for behaviour video footage, together with time stamps and integrated control



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Descriptions

The NINscope

Creative tinkerers and developers, along with recent advances in optogenetics, genetically-encoded Calcium indicators and miniaturized optics and electronics made head-mountable microscopes a popular tool. Innovations from the founders of Inscopix and the UCLA team with their Miniscope have made this technology more accessible.

The NINscope (developed and published by de Groot et al., 2019 from the Netherlands Institute for Neuroscience) is a more recent development. It is a  smaller footprint, light weight hybrid of the Miniscope, with several other interesting integrated features. It is ideal for use on small freely behaving animals, or when two simultaneous recording areas is of interest, since it is small enough for a mouse to have two NINscope's mounted at the same time.  

NINscope design and features

For more information on miniscope designs and features, please read further in the publication by de Groot et al., 2019. They described their instrument design, manufacturing methods and functionality in detail here.

NINscope Specifications

Weight - 1.6g (Yes, this includes the housing, optics & PCBs!)

Footprint -6.5 x 7.5 mm baseplate 

Sensor - CMOS 752x480 pixels (Python480)  with Kyocera CMOS clock oscillator  

Pixel Size - 4.8 µm  

Inertial Measurement Unit - Yes, 3D accelerometer (STMicoreectronics LSM6DSLTR) 

LED Driver - Yes, 2 LED drivers and 12C I/O expander  

Accuracy -1 ms 

Optogenetic stimulation LED probe - Yes, custom probe 

Multi-region recordings - Yes 

Inter-baseplate distance - 8.15 mm 

Wireless - Yes

Coaxial Cable - CMOS connects via FPD-Link III and coaxial to USB controller on the DAQ board 

Excitation/Emission Filters size - 3.5x3.5x0.5 mm Excitation/ 4x4x1 mm Emission 

Dichroic Mirror - Chroma T495lpxr  (3.5x5x0.5 mm) 

GRIN lens - Yes, #64-519 Edmund Optics Grin lens with 1.8 mm diameter *implanted or glued into baseplate 

Numerical Aperture - 0.55

Magnification - 4.6x

DAQ - UCLA Miniscope DAQ card with minor modifications

Time per recording session - 2-11 min with brief intermissions 

Photobleaching/damage - None evident

Enclosure - 3D printed

Open Source Software - Yes, platform independent user interface with two channels for recordings and an additional USB webcam for behaviour video footage, together with time stamps and integrated control




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